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Measuring speed is a critical factor to reduce motion artifacts for dynamic scene capture. Phase-shifting methods have the advantage of providing high-accuracy and dense 3D point clouds, but the phase unwrapping process affects the measurement speed. This paper presents an absolute phase unwrapping method capable of using only three speckle-embedded phase-shifted patterns for high-speed three-dimensional (3D) shape measurement on a single-camera, single-projector structured light system. The proposed method obtains the wrapped phase of the object from the speckle-embedded three-step phase-shifted patterns. Next, it utilizes the Semi-Global Matching (SGM) algorithm to establish the coarse correspondence between the image of the object with the embedded speckle pattern and the pre-obtained image of a flat surface with the same embedded speckle pattern. Then, a computational framework uses the coarse correspondence information to determine the fringe order pixel by pixel. The experimental results demonstrated that the proposed method can achieve high-speed and high-quality 3D measurements of complex scenes. 

This paper presents an absolute phase unwrapping method for high-speed three-dimensional (3D) shape measurement. This method uses three phase-shifted patterns and one binary random pattern on a single-camera, single-projector structured light system. We calculate the wrapped phase from phase-shifted images and determine the coarse correspondence through the digital image correlation (DIC) between the captured binary random pattern of the object and the pre-captured binary random pattern of a flat surface. We then developed a computational framework to determine fringe order number pixel by pixel using the coarse correspondence information. Since only one additional pattern is used, the proposed method can be used for high-speed 3D shape measurement. Experimental results successfully demonstrated that the proposed method can achieve high-speed and high-quality measurement of complex scenes. 

Sepsis is a lethal syndrome manifested by an unregulated, overwhelming inflammation from the host in response to infection. Here, we exploit the use of a synthetic heparan sulfate octadecasaccharide (18-mer) to protect against sepsis. The 18-mer not only inhibits the pro-inflammatory activity of extracellular histone H3 and high mobility group box 1 (HMGB1), but also elicits the anti-inflammatory effect from apolipoprotein A-I (ApoA-I). We demonstrate that the 18-mer protects against sepsis-related injury and improves survival in cecal ligation and puncture mice and reduces inflammation in an endotoxemia mouse model. The 18-mer neutralizes the cytotoxic histone-3 (H3) through direct interaction with the protein. Furthermore, the 18-mer enlists the actions of ApoA-I to dissociate the complex of HMGB1 and lipopolysaccharide, a toxic complex contributing to cell death and tissue damage in sepsis. Our study provides strong evidence that the 18-mer mitigates inflammatory damage in sepsis by targeting numerous mediators, setting it apart from other potential therapies with a single target. 

Abstract The organization of chromatin into self-interacting domains is universal among eukaryotic genomes, though how and why they form varies considerably. Here we report a chromosome-scale reference genome assembly of pepper ( Capsicum annuum ) and explore its 3D organization through integrating high-resolution Hi-C maps with epigenomic, transcriptomic, and genetic variation data. Chromatin folding domains in pepper are as prominent as TADs in mammals but exhibit unique characteristics. They tend to coincide with heterochromatic regions enriched with retrotransposons and are frequently embedded in loops, which may correlate with transcription factories. Their boundaries are hotspots for chromosome rearrangements but are otherwise depleted for genetic variation. While chromatin conformation broadly affects transcription variance, it does not predict differential gene expression between tissues. Our results suggest that pepper genome organization is explained by a model of heterochromatin-driven folding promoted by transcription factories and that such spatial architecture is under structural and functional constraints. 

The rapid evolution of repetitive DNA sequences, including satellite DNA, tandem duplications, and transposable elements, underlies phenotypic evolution and contributes to hybrid incompatibilities between species. However, repetitive genomic regions are fragmented and misassembled in most contemporary genome assemblies. We generated highly contiguous de novo reference genomes for the Drosophila simulans species complex ( D. simulans , D. mauritiana , and D. sechellia ), which speciated ∼250,000 yr ago. Our assemblies are comparable in contiguity and accuracy to the current D. melanogaster genome, allowing us to directly compare repetitive sequences between these four species. We find that at least 15% of the D. simulans complex species genomes fail to align uniquely to D. melanogaster owing to structural divergence—twice the number of single-nucleotide substitutions. We also find rapid turnover of satellite DNA and extensive structural divergence in heterochromatic regions, whereas the euchromatic gene content is mostly conserved. Despite the overall preservation of gene synteny, euchromatin in each species has been shaped by clade- and species-specific inversions, transposable elements, expansions and contractions of satellite and tRNA tandem arrays, and gene duplications. We also find rapid divergence among Y-linked genes, including copy number variation and recent gene duplications from autosomes. Our assemblies provide a valuable resource for studying genome evolution and its consequences for phenotypic evolution in these genetic model species.